HLA Typing pp 89-100 | Cite as

High-Throughput Contiguous Full-Length Next-Generation Sequencing of HLA Class I and II Genes from 96 Donors in a Single MiSeq Run

  • Philip K. Ehrenberg
  • Aviva Geretz
  • Rasmi ThomasEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1802)


The human leukocyte antigen (HLA) genes regulate and drive the immune system, and are among the most polymorphic loci in the human genome. HLA diversity is known to play an important role in transplantation and disease association studies. There are multiple approaches to DNA-based HLA genotyping and recent advances in next-generation sequencing (NGS) technologies have facilitated the development of whole gene sequencing methods. We describe an accurate, efficient, scalable, and cost-effective approach to contiguously amplify and sequence full-length genes of six HLA class I and II loci from 96 individuals on a single Illumina MiSeq run.


HLA HLA genotyping NGS Next-generation sequencing Full-length amplification MIT-NGS Illumina MiSeq 



This work was supported by a cooperative agreement (W81XWH-07-2-0067) between the Henry M. Jackson Foundation for the Advancement of Military Medicine, Inc., and the U.S. Department of Defense (DOD). This research was funded, in part, by the U.S. National Institute of Allergy and Infectious Disease. The views expressed are those of the authors and should not be construed to represent the positions of the U.S. Army or the DOD.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Philip K. Ehrenberg
    • 1
  • Aviva Geretz
    • 1
    • 2
  • Rasmi Thomas
    • 1
    • 2
    Email author
  1. 1.U.S. Military HIV Research Program (MHRP)Walter Reed Army Institute of ResearchSilver SpringUSA
  2. 2.Henry M. Jackson Foundation for the Advancement of Military Medicine (HJF)BethesdaUSA

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