HLA Typing pp 225-233 | Cite as

Comprehensive HLA Typing from a Current Allele Database Using Next-Generation Sequencing Data

  • Shuji Kawaguchi
  • Koichiro Higasa
  • Ryo Yamada
  • Fumihiko MatsudaEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1802)


HLA allele information is essential for a variety of medical applications, such as genomic studies of multifactorial diseases, including immune system and inflammation-related disorders, and donor selection in organ transplantation and regenerative medicine. To obtain this information, an accurate HLA typing method that is applicable for any allele registered in HLA allele databases is needed. Here, we describe a method for determining alleles from a current HLA database using next-generation sequencing (NGS) results.


HLA typing NGS Software Database Bioinformatics 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Shuji Kawaguchi
    • 1
  • Koichiro Higasa
    • 1
  • Ryo Yamada
    • 1
  • Fumihiko Matsuda
    • 1
    Email author
  1. 1.Center for Genomic MedicineKyoto University Graduate School of MedicineKyotoJapan

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