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Quantification of Thyromimetic Sobetirome Concentration in Biological Tissue Samples

  • Jordan Devereaux
  • Skylar J. Ferrara
  • Thomas S. Scanlan
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1801)

Abstract

Thyroid hormone is a principal regulator of essential processes in vertebrate physiology and homeostasis. Synthetic derivatives of thyroid hormone, known as thyromimetics, display desirable therapeutic properties. Thoroughly understanding how thyromimetics distribute throughout the body is crucial for their development and this requires appropriate bioanalytical techniques to quantify drug levels in different tissues. Here, we describe a detailed protocol for the quantification of the thyromimetic sobetirome using liquid chromatography tandem-mass spectrometry (LC-MS/MS).

Key words

Sobetirome Thyromimetic Thyroid hormone LC-MS/MS Drug quantification Pharmacokinetics PK-ADME 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Jordan Devereaux
    • 1
  • Skylar J. Ferrara
    • 1
  • Thomas S. Scanlan
    • 1
  1. 1.Program in Chemical Biology, Department of Physiology & PharmacologyOregon Health & Science UniversityPortlandUSA

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