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Evaluating Thyroid Disrupting Chemicals In Vivo Using Xenopus laevis

  • Bilal B. Mughal
  • Barbara A. Demeneix
  • Jean-Baptiste Fini
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1801)

Abstract

Using in vivo animal model systems for chemical screening can permit evaluation of the signaling pathways implicated. Xenopus laevis is an ideal model organism to test thyroid axis disruption as thyroid hormones are highly conserved across vertebrates. Here, we describe a high-throughput assay using non-feeding embryonic stage transgenic X. laevis (TH/bZip) to screen for thyroid disrupting chemicals using a 3 day exposure protocol. We further describe a protocol to detect endocrine disruption of thyroid axis by the analysis of gene expression using wild-type X. laevis.

Key words

Xenopus laevis Thyroid signaling Endocrine disruption Thyroid disruption XETA assay Neurodevelopment Gene expression Behavior analysis 

Notes

Acknowledgments

We thank Gérard Benisti, Philippe Durand and Jean-Paul Chaumeil for excellent animal care and thank Sébastien Le Mével for his input in the methods which are routinely used. This protocol has been refined thanks to work supported by grants from Centre National de la Recherche Scientifique (CNRS), Muséum National d’Histoire Naturelle (MNHN), and from European Union DevCom FP7-People-2013-ITN N°607142.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Bilal B. Mughal
    • 1
  • Barbara A. Demeneix
    • 1
  • Jean-Baptiste Fini
    • 1
  1. 1.Evolution des Régulations Endocriniennes, Département “Adaptation du Vivant”UMR 7221 Muséum National d’Histoire Naturelle /CNRSParisFrance

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