The Use of CRISPR-Cas9 Technology to Reveal Important Aspects of Human Airway Biology
The CRISPR-Cas9 technology is a powerful tool that enables site-specific genome modification (gene editing) and is increasingly used in research to generate gene knockout or knock-in in a variety of cells and organisms. This chapter provides a brief overview of this technology and describes a general methodology applicable to human airway biology research.
Key wordsCRISPR-Cas9 Gene editing Lentivirus Lung Airway epithelium Primary cells
This work was supported by the following grants from NIH: 1U19AI125357, R01HL122321, R01AI106287, and R01HL125128.
The authors wish to thank Max Seibold, Jamie Everman, and Ari Stoner (Dr. Max Seibold’s Lab, National Jewish Health, Denver) for technical advice and support.
- 12.Bauer DE, Canver MC, Orkin SH (2015) Generation of genomic deletions in mammalian cell lines via CRISPR/Cas9. J Vis Exp 95:e52118Google Scholar