Assessing the Mouse Intestinal Microbiota in Settings of Type-2 Immune Responses
The microbial communities that reside within the mammalian host play important roles in the development of a robust host immune system. With the advent of sequencing technology and barcoding strategy of the bacterial 16S ribosomal RNA (rRNA) gene, microbiota studies are becoming more economical but also more important in many immunology studies. Here, we described a representative study protocol to characterize how the microbiota changes during an intestinal helminth infection, with emphasis on subtle aspects of the experimental design that are critical for data interpretation.
Key words16S rRNA Microbiota Helminth Type 2 immunity
We thank Laurie M. Cox and the Blaser lab at the New York University School of Medicine for assistance with setting up 16S rRNA sequencing in our laboratory and for providing the reverse barcoded primer constructs. We thank David Artis for seed stock of T. muris.
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