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Isolation and Screening of Cellulose-Degrading Microorganisms from Different Ecological Niches

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Cellulases

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1796))

Abstract

Increased interest in developing cellulose-based ethanol over the last few years was the main reason behind inflated research to find cellulose-degrading microorganisms. Several methods have been developed in the past for efficient isolation and characterization of cellulolytic microorganisms. However, it is critical to choose a specific method from a list of qualitative methods for the characterization of cellulose degrading microorganisms. In this chapter, we have extensively listed various qualitative methods used for the isolation and characterization of the cellulolytic microorganisms isolated from different ecological niches such as soil, decaying wood, gut, and rumen.

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References

  1. McDonald JE, Rooks DJ, McCarthy AJ (2012) Methods for the isolation of cellulose-degrading microorganisms. Methods Enzymol 510:349–374

    Article  CAS  Google Scholar 

  2. Lynd LR, Weimer PJ, Van Zyl WH, Pretorius IS (2002) Microbial cellulose utilization: fundamentals and biotechnology. Microbiol Mol Biol Rev 66:506–577

    Article  CAS  Google Scholar 

  3. Saloheimo M, Pakula TM (2012) The cargo and the transport system: secreted proteins and protein secretion in Trichoderma reesei (Hypocrea jecorina). Microbiology 158:46–57

    Article  CAS  Google Scholar 

  4. Gilbert HJ (2007) Cellulosomes: microbial nanomachines that display plasticity in quaternary structure. Mol Microbiol 63:1568–1576

    Article  CAS  Google Scholar 

  5. Yanke L, Cheng K (1998) A method for the selective enumeration and isolation of ruminal Lactobacillus and Streptococcus. Lett Appl Microbiol 26:248–252

    Article  CAS  Google Scholar 

  6. Kasana RC, Salwan R, Dhar H et al (2008) A rapid and easy method for the detection of microbial cellulases on agar plates using Gram’s iodine. Curr Microbiol 57:503–507

    Article  CAS  Google Scholar 

  7. Pointing SB (1999) Qualitative methods for the determination of lignocellulolytic enzyme production by tropical fungi. Fungal Div 2:17–33

    Google Scholar 

  8. Rautela GS, Cowling EB (1966) Simple cultural test for relative cellulolytic activity of fungi. Appl Microbiol 14:892–898

    CAS  PubMed  PubMed Central  Google Scholar 

  9. Egger KN (1986) Substrate hydrolysis patterns of post-fire ascomycetes (Pezizales). Mycologia 78:771–780

    Article  CAS  Google Scholar 

  10. Rohrmann S, Molitoris H-P (1992) Screening for wood-degrading enzymes in marine fungi. Can J Bot 70:2116–2123

    Article  CAS  Google Scholar 

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Correspondence to Wensheng Qin .

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Kameshwar, A.K.S., Qin, W. (2018). Isolation and Screening of Cellulose-Degrading Microorganisms from Different Ecological Niches. In: Lübeck, M. (eds) Cellulases. Methods in Molecular Biology, vol 1796. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7877-9_4

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  • DOI: https://doi.org/10.1007/978-1-4939-7877-9_4

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7876-2

  • Online ISBN: 978-1-4939-7877-9

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