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Two-Hybrid Systems pp 61–73Cite as

The Ras Recruitment System (RRS) for the Identification and Characterization of Protein–Protein Interactions

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1794))

Abstract

Protein–protein interactions are the basis for all biochemical cellular activities. The Ras Recruitment System, RRS, is a method for studying interactions between known proteins as well as identification of novel interactions following a cDNA library screen. The method is based on the recruitment of the Ras protein to the plasma membrane via protein–protein interactions. The interaction between proteins is studied in a temperature-sensitive yeast Saccharomyces cerevisiae mutant strain. This mutant is able to grow under restrictive temperature conditions when the Ras viability pathway becomes activated as a result of a positive protein–protein interaction. The RRS complements the limitations and problems that arise from the yeast two-hybrid system.

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Acknowledgments

The author thanks Drs. Broder Y., Katz S., Maroun M., Cohen A., and all Aronheim’s lab alumni for the continued development of the protein recruitment systems over the years.

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Authors and Affiliations

  1. Department of Cell Biology and Cancer Science, The B. Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa, Israel

    Ami Aronheim

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  1. Ami Aronheim
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Correspondence to Ami Aronheim .

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Editors and Affiliations

  1. Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid (UPM)–Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Campus Montegancedo UPM, Madrid, Spain

    Luis Oñate-Sánchez

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Aronheim, A. (2018). The Ras Recruitment System (RRS) for the Identification and Characterization of Protein–Protein Interactions. In: Oñate-Sánchez, L. (eds) Two-Hybrid Systems. Methods in Molecular Biology, vol 1794. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7871-7_5

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  • DOI: https://doi.org/10.1007/978-1-4939-7871-7_5

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7870-0

  • Online ISBN: 978-1-4939-7871-7

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