Abstract
Mass cytometry has emerged as a new state-of-the-art technology that enables in-depth characterization of cellular populations and functions at a single cell resolution. We describe the application of this technology to deeply phenotype the blood and bone marrow components of multiple myeloma patients in a clinical setting. This technology allows for simultaneous quantification of more than 40 markers, overcoming the challenges of traditional fluorescence-based flow cytometry.
Key words
- Mass cytometry
- CyTOF
- Multiple myeloma
- Immunophenotyping
- Single cell
- Barcoding
- Smart tube
- Peripheral blood
- Bone marrow
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Change history
07 February 2019
In Chapter 4, the author’s name Homer Adams III has been modified to Adams HC 3rd, as requested by him.
References
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Acknowledgments
The authors would like to thank Christoph Heuck for his suggestions and critical reading of the manuscript.
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Smets, T., Stevenaert, F., Adams, H., Vanhoof, G. (2018). Deep Profiling of the Immune System of Multiple Myeloma Patients Using Cytometry by Time-of-Flight (CyTOF). In: Heuck, C., Weinhold, N. (eds) Multiple Myeloma. Methods in Molecular Biology, vol 1792. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-7865-6_4
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DOI: https://doi.org/10.1007/978-1-4939-7865-6_4
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