Abstract
Positron emission tomography (PET) is a three dimensional imaging modality that detects the accumulation of radiolabeled isotopes in vivo. Ectopic expression of a thymidine kinase reporter gene allows for the specific detection of reporter cells in vivo by imaging with the reporter specific probe. PET reporter imaging is sensitive, quantitative and can be scaled into larger tumors or animals with little to no tissue diffraction. Here, we describe how thymidine kinase PET reporter genes can be used to noninvasively image cancer cells in vivo.
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Acknowledgment
M.N.M. is supported by the Stanford Dean’s Fellowship and the A.P. Giannini Foundation.
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McCracken, M.N. (2018). Thymidine Kinase PET Reporter Gene Imaging of Cancer Cells In Vivo. In: Dubey, P. (eds) Reporter Gene Imaging. Methods in Molecular Biology, vol 1790. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7860-1_11
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DOI: https://doi.org/10.1007/978-1-4939-7860-1_11
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