Skip to main content

Vacuolar Sorting Determinants: Isolation and Study

  • Protocol
  • First Online:
Plant Vacuolar Trafficking

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1789))

  • 1038 Accesses

Abstract

As it serves an important function in the compartmentalization of a series of components, from secondary metabolites to proteins, the vacuole is a central organelle in plant cell biology and development. One of the most important mechanisms regulating not only vacuolar biogenesis but also its luminal content, is the vacuolar sorting of proteins. This sorting mechanism is based upon the recognition of specific signal sequences, vacuolar sorting determinants, by sorting receptors, which then act by redirecting vacuolar cargo away from the default secretory pathway, and into the vacuolar lumen. One of the most direct ways of understanding if a given peptide possesses vacuolar sorting determinant capability is to isolate it, fuse it with a marker and express it in a plant system such as Nicotiana tabacum, a pipeline which will be described in more detail in this chapter.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Similar content being viewed by others

References

  1. Zhang C, Hicks GR, Raikhel NV (2014) Plant vacuolar morphology and vacuolar trafficking. Front Plant Sci 5:476–485

    PubMed  PubMed Central  Google Scholar 

  2. Peixoto B, Pereira S, Pissarra J (2016) Plant vacuolar sorting: an overview. In: Cánovas FM, Lüttge U, Matyssek R (eds) Progress in botany, vol 78. Springer International Publishing, Cham, p 67

    Google Scholar 

  3. de Marcos LC, Denecke J (2016) Lysosomal and vacuolar sorting: not so different after all! Biochem Soc Trans 44:891–897

    Article  CAS  Google Scholar 

  4. Neuhaus JM, Rogers JC (1998) Sorting of proteins to vacuoles in plant cells. Plant Mol Biol 38:127–144

    Article  CAS  PubMed  Google Scholar 

  5. Pereira C, Pereira S, Satiat-Jeunemaitre B, Pissarra J (2013) Cardosin A contains two vacuolar sorting signals using different vacuolar routes in tobacco epidermal cells. Plant J 76:87–100

    PubMed  CAS  Google Scholar 

  6. Jolliffe NA, Craddock CP, Frigerio L (2005) Pathways for protein transport to seed storage vacuoles. Biochem Soc Trans 33:1016–1018

    Article  CAS  PubMed  Google Scholar 

  7. Zouhar J, Rojo E (2009) Plant vacuoles: where did they come from and where are they heading? Curr Opin Plant Biol 12:677–684

    Article  CAS  PubMed  Google Scholar 

  8. Batoko H, Zheng HQ, Hawes C, Moore I (2000) A rab1 GTPase is required for transport between the endoplasmic reticulum and golgi apparatus and for normal golgi movement in plants. Plant Cell 12(11):2201–2218

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Littlejohn G, Love J (2012) A simple method for imaging Arabidopsis leaves using perfluorodecalin as an infiltrative imaging medium. J Vis Exp 59:e3394. https://doi.org/10.3791/3394

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Susana Pereira .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2018 Springer Science+Business Media, LLC, part of Springer Nature

About this protocol

Check for updates. Verify currency and authenticity via CrossMark

Cite this protocol

Peixoto, B., Pereira, S., Pereira, C., Pissarra, J. (2018). Vacuolar Sorting Determinants: Isolation and Study. In: Pereira, C. (eds) Plant Vacuolar Trafficking. Methods in Molecular Biology, vol 1789. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7856-4_3

Download citation

  • DOI: https://doi.org/10.1007/978-1-4939-7856-4_3

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7855-7

  • Online ISBN: 978-1-4939-7856-4

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics