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Phenotypic Screening of Small Molecules with Antimalarial Activity for Three Different Parasitic Life Stages

  • Nobutaka Kato
  • Sandra March
  • Sangeeta N. Bhatia
  • Matthias Marti
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1787)

Abstract

Malaria remains one of the deadliest infectious diseases globally. Available therapeutic agents are already limited in their efficacy, and drug resistance threatens to diminish further our ability to prevent and treat the disease. Despite a renewed effort to identify compounds with antimalarial activity, the drug discovery and development pipeline lacks target diversity and availability of compounds that target liver- and gametocyte-stage parasites. Phenotypic screens are a powerful and valuable tool for identifying new chemical compounds with antimalarial activity. This chapter highlights recent phenotypic screening methodologies for all three parasitic life stages.

Key words

Malaria Plasmodium falciparum Asexual blood-stage Liver-stage Gametocyte-stage Phenotypic screening 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Nobutaka Kato
    • 1
  • Sandra March
    • 1
    • 2
    • 3
    • 4
  • Sangeeta N. Bhatia
    • 1
    • 2
    • 3
    • 4
  • Matthias Marti
    • 5
    • 6
  1. 1.Broad Institute of MIT and HarvardCambridgeUSA
  2. 2.Institute for Medical Engineering and Science, Massachusetts Institute of TechnologyCambridgeUSA
  3. 3.Howard Hughes Medical InstituteCambridgeUSA
  4. 4.Koch Institute for Integrative Cancer ResearchCambridgeUSA
  5. 5.Wellcome Centre for Molecular Parasitology, University of GlasgowGlasgowUK
  6. 6.Department of Immunology and Infectious DiseasesHarvard TH Chan School of Public HealthBostonUSA

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