Techniques to Enable 1536-Well Phenotypic Screening

  • Sinéad Knight
  • Helen Plant
  • Lisa McWilliams
  • Mark Wigglesworth
Part of the Methods in Molecular Biology book series (MIMB, volume 1787)


Adaptation of phenotypic cell assays to 1536-well format brings major challenges in liquid handling for high-content assays requiring washing steps and coating of plates. In addition, problematic edge effects and reduced assay quality are frequently encountered. In this chapter, we describe the novel application of a centrifugal plate washer to facilitate miniaturization of 1536-well cell assays and a combination of techniques to reduce edge effects, all of which improved throughput and data quality. Cell assays currently limited in throughput because of cost and complex protocols may be enabled by the techniques presented in this chapter.

Key words

Miniaturization 1536-well High-throughput Cell assays Phenotypic screening 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Sinéad Knight
    • 1
  • Helen Plant
    • 2
  • Lisa McWilliams
    • 1
  • Mark Wigglesworth
    • 2
  1. 1.Discovery Sciences, IMED Biotech UnitAstraZenecaCambridgeUK
  2. 2.Discovery Sciences, IMED Biotech UnitAstraZenecaMacclesfieldUK

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