Phenotype-Based High-Content Screening Using Fluorescent Chemical Bioprobes: Lipid Droplets and Glucose Uptake Quantification in Live Cells

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1787)

Abstract

Phenotypic screening in live cells has emerged as a promising strategy for drug discovery in pharmaceutical communities. For relevant phenotype-based screening setups, it is critical to develop adequate reporters in order to selectively visualize subcellular compartments or phenotypic changes that represent disease-related characteristics during compound screening. In this chapter, we introduce two phenotype-based high-content/high-throughput assays using fluorescent bioprobes that have been designed and refined to selectively stain cellular lipid droplets (LDs) and to show cellular glucose uptake. In conjunction with target identification process for the hit compounds from phenotypic screening, these fluorescent chemical probe-based screening techniques are expected to drive a great advancement for the discovery of novel first-in-class therapeutics.

Key words

Phenotypic screening Image-based screening Fluorescent bioprobe Seoul-Fluor 44 (SF44) Glucose bioprobe (GB2-Cy3) 

Notes

Acknowledgment

This work was supported by the National Creative Research Initiative Grant (NRF-2014R1A3A2030423) and the Bio & Medical Technology Development Program (2012M3A9C4048780) through the National Research Foundation of Korea (NRF) funded by the Korean Government (Ministry of Science and ICT​). This research was also supported by Basic Science Research Program (NRF-2009-0093826, 2016R1A6A3A01011351) through NRF funded by the Korean Ministry of Education.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of ChemistrySeoul National UniversitySeoulSouth Korea
  2. 2.Department of Biophysics and Chemical BiologySeoul National UniversitySeoulSouth Korea

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