Abstract
We present a method to study the interaction between biomolecules and receptors present on the cell surface. This enables studies of molecular interactions in a natural biological context. As the analyte interacts with the receptors still intact on the cell surface, the experimental data provides complete dynamics and complexity of the interaction, thereby generating highly informative data. Attana’s cell-based biosensor platform can be used to obtain this information from a diverse range of interactions as described in these protocols, which detail how to grow or capture cells on a surface, how to stabilize and visualize the cells on the surface, and how to set up assays to measure detailed interaction kinetics directly on the cell surface.
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Peiris, D. et al. (2018). Label-Free Cell-Based Assay for Characterization of Biomolecules and Receptors. In: Rockberg, J., Nilvebrant, J. (eds) Epitope Mapping Protocols. Methods in Molecular Biology, vol 1785. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7841-0_4
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DOI: https://doi.org/10.1007/978-1-4939-7841-0_4
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7839-7
Online ISBN: 978-1-4939-7841-0
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