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The Importance of Experimental Design, Quality Assurance, and Control in Plant Metabolomics Experiments

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Book cover Plant Metabolomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1778))

Abstract

The output of metabolomics relies to a great extent upon the methods and instrumentation to identify, quantify, and access spatial information on as many metabolites as possible. However, the most modern machines and sophisticated tools for data analysis cannot compensate for inappropriate harvesting and/or sample preparation procedures that modify metabolic composition and can lead to erroneous interpretation of results. In addition, plant metabolism has a remarkable degree of complexity, and the number of identified compounds easily surpasses the number of samples in metabolomics analyses, increasing false discovery risk. These aspects pose a large challenge when carrying out plant metabolomics experiments. In this chapter, we address the importance of a proper experimental design taking into consideration preventable complications and unavoidable factors to achieve success in metabolomics analysis. We also focus on quality control and standardized procedures during the metabolomics workflow.

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Acknowledgments

C. Caldana was funded by the São Paulo Research Foundation (FAPESP) grant no. 2012/19561-0 and the Max Planck Society.

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Author notes

  1. Camila Caldana

    Present address: Max Planck Institute of Molecular Plant Physiology, Potsdam-Golm, Germany

Authors and Affiliations

  1. Brazilian Bioethanol Science and Technology Laboratory (CTBE), Brazilian Center for Research in Energy and Materials (CNPEM), Campinas, São Paulo, Brazil

    Marina C. M. Martins, Camila Caldana, Lucia Daniela Wolf & Luis Guilherme Furlan de Abreu

  2. Max-Planck Partner Group, Brazilian Bioethanol Science and Technology Laboratory (CTBE/CNPEM), Campinas, Brazil

    Camila Caldana

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  1. Marina C. M. Martins
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  2. Camila Caldana
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  3. Lucia Daniela Wolf
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  4. Luis Guilherme Furlan de Abreu
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Correspondence to Camila Caldana .

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Editors and Affiliations

  1. Plant Metabolomics Laboratory, Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa (ITQB NOVA), Oeiras, Portugal

    Carla António

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Martins, M.C.M., Caldana, C., Wolf, L.D., de Abreu, L.G.F. (2018). The Importance of Experimental Design, Quality Assurance, and Control in Plant Metabolomics Experiments. In: António, C. (eds) Plant Metabolomics. Methods in Molecular Biology, vol 1778. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7819-9_1

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