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Luminescent-Conjugated Oligothiophene Probe Applications for Fluorescence Imaging of Pure Amyloid Fibrils and Protein Aggregates in Tissues

  • K. Peter R. Nilsson
  • Mikael Lindgren
  • Per Hammarström
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1779)

Abstract

Luminescent-conjugated oligo- and polythiophenes (LCOs and LCPs) are valuable tools for optical imaging of a plethora of protein aggregates associated with amyloidoses. Here, we outline updated protocols for the application of the anionic pentameric LCO, p-FTAA, for staining and hyperspectral imaging of protein aggregates in a variety of settings such as in vitro formed amyloid fibrils, ex vivo tissue sections, and whole brain Drosophila.

Key words

Luminescent-conjugated oligothiophenes Protein aggregates Fluorescence imaging p-FTAA Amyloid 

Notes

Acknowledgment

This work was supported by the Swedish Research Council (P.H.), Linköping University (P.H., K.P.R.N., M.L.), The Swedish Foundation for Strategic Research (K.P.R.N.), and Göran Gustafsson Foundation (P.H.). We thank Maria Jonson for the spectral image of Drosophila brain and Daniel Sjölander for the images of TTR cardiac amyloid.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • K. Peter R. Nilsson
    • 1
  • Mikael Lindgren
    • 1
    • 2
  • Per Hammarström
    • 1
  1. 1.IFM-Department of ChemistryLinköping UniversityLinköpingSweden
  2. 2.Department of PhysicsThe Norwegian University of Science and TechnologyTrondheimNorway

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