Mapping Amyloid Regions in Gad m 1 with Peptide Arrays
Amyloid formation is basically featured by a protein-protein interaction in which the reacting regions are the segments assembling into cross β-sheets. To identify these segments both theoretical and experimental tools have been developed. Here, we focus on the use of peptide arrays to probe the binding of several amyloid-specific probes such as the OC and A11 anti-amyloid conformation-selective antibodies and of monomers and preformed fibrils. These arrays use libraries containing partly overlapping peptides derived from the sequence of Gad m 1, the major allergen from Atlantic cod, which forms amyloids under gastrointestinal relevant conditions.
Key wordsPeptide arrays Amyloids Anti-amyloid antibodies Food allergens Gad m 1
This work was supported by grants from Spanish AEI/EU-FEDER SAF2014-52661-C3 and BFU2015-72271-EXP. M.C. was supported by a Juan de la Cierva Postdoctoral contract.
- 26.Martinez J, Sanchez R, Castellanos M et al (2015) Fish beta-parvalbumin acquires allergenic properties by amyloid assembly. Swiss Med Weekly 145:w14128Google Scholar