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Mapping Amyloid Regions in Gad m 1 with Peptide Arrays

Protocol
First Online:
Part of the Methods in Molecular Biology book series (MIMB, volume 1779)

Abstract

Amyloid formation is basically featured by a protein-protein interaction in which the reacting regions are the segments assembling into cross β-sheets. To identify these segments both theoretical and experimental tools have been developed. Here, we focus on the use of peptide arrays to probe the binding of several amyloid-specific probes such as the OC and A11 anti-amyloid conformation-selective antibodies and of monomers and preformed fibrils. These arrays use libraries containing partly overlapping peptides derived from the sequence of Gad m 1, the major allergen from Atlantic cod, which forms amyloids under gastrointestinal relevant conditions.

Key words

Peptide arrays Amyloids Anti-amyloid antibodies Food allergens Gad m 1 
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Notes

Acknowledgments

This work was supported by grants from Spanish AEI/EU-FEDER SAF2014-52661-C3 and BFU2015-72271-EXP. M.C. was supported by a Juan de la Cierva Postdoctoral contract.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Institute of Physical Chemistry “Rocasolano”Spanish National Research Council (CSIC)MadridSpain
  2. 2.Faculdade de Ciências, Departamento de Química e Bioquímica, Biosystems and Integrative Sciences InstituteUniversidade de LisboaLisbonPortugal
  3. 3.Departamento de Química e BioquímicaUniversidade de LisboaLisbonPortugal
  4. 4.IMDEA NacoscienceMadridSpain

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