Imaging Protein Fibers at the Nanoscale and In Situ
Protein self-assembly offers a rich repertoire of tools and technologies. However, despite significant progress in this area, a deterministic measure of the phenomenon, which might lead to predictable relationships between protein components, assembly mechanisms, and ultimately function, is lacking. Often the challenge relates to the choice of the most informative and precise measurements that can link the chemistry of the building blocks with the resulting assembly, ideally in situ and in real time. Using the example of protein fibrillogenesis—a self-assembly process fundamental to nearly every aspect of biological organization, from viral assembly to tissue restoration—this chapter demonstrates how protein self-assembly can be visually and precisely measured while providing measurement protocols applicable to other self-assembly systems.
Key wordsMolecular self-assembly Nanometrology Real-time imaging Super-resolution microscopy Nanoscale biophysics Protein fibrillogenesis
- 5.Hay EH (2013) Cell biology of extracellular matrix, 2nd edn. Springer Science & Business Media, DordrechtGoogle Scholar
- 25.Hartmann MD, Mendler CT, Bassler J, Karamichali I, Ridderbusch O, Lupas AN, Avarez BH (2016) α/β coiled coils. elife 5:11861Google Scholar
- 39.Kuipers BJH, Gruppen H (2007) Prediction of molar extinction coefficients of proteins and peptides using UV absorption of the constituent amino acids at 214 nm to enable quantitative reverse phase high-performance liquid chromatography-mass spectrometry analysis. J Agric Food Chem 55:5445–5451CrossRefGoogle Scholar