Experimental and Computational Protocols for Studies of Cross-Seeding Amyloid Assemblies

  • Baiping Ren
  • Rundong Hu
  • Mingzhen Zhang
  • Yonglan Liu
  • Lijian Xu
  • Binbo Jiang
  • Jie Ma
  • Buyong Ma
  • Ruth Nussinov
  • Jie Zheng
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1777)

Abstract

Alzheimer’s disease (AD) and type 2 diabetes (T2D) are two common protein aggregation diseases. Compelling evidence has shown a link between AD and T2D, which may derive from interspecies cross-sequence interactions between amyloid-β peptide (Aβ), associated with AD, and human islet amyloid polypeptide (hIAPP), associated with T2D. Herein, we present experimental and computational protocols and tools to study the aggregate structures and kinetics, conformational conversion, and molecular interactions of Aβ-hIAPP mixtures. These protocols could be generally applied to other cross-seeding behaviors of amyloid peptides.

Key words

Amyloid peptides Aβ hIAPP Cross-seeding Alzheimer disease Diabetes 

Notes

Acknowledgments

J.Z. thanks the financial support from NSF (CBET-1510099 and DMR-1607475), Alzheimer Association (2015-NIRG-341372), and National Natural Science Foundation of China (NSFC-21528601). The high-performance computational facilities of the Biowulf PC/Linux cluster at the NIH were mainly used for the simulations. This project has been funded in whole or in part with Federal funds from the National Cancer Institute, National Institutes of Health, under contract number HHSN261200800001E. The content of this publication does not necessarily reflect the views or policies of the Department of Health and Human Services, nor does mention of trade names, commercial products, or organizations imply endorsement by the U.S. Government. This research was supported (in part) by the Intramural Research Program of the NIH, National Cancer Institute, Center for Cancer Research.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Baiping Ren
    • 1
  • Rundong Hu
    • 1
  • Mingzhen Zhang
    • 1
  • Yonglan Liu
    • 1
  • Lijian Xu
    • 1
    • 2
  • Binbo Jiang
    • 1
    • 3
  • Jie Ma
    • 1
    • 4
  • Buyong Ma
    • 5
  • Ruth Nussinov
    • 5
    • 6
  • Jie Zheng
    • 1
  1. 1.Department of Chemical & Biomolecular EngineeringThe University of AkronAkronUSA
  2. 2.College of Life Sciences and Chemistry Hunan University of TechnologyZhuzhouChina
  3. 3.College of Chemical and Biological EngineeringZhejiang UniversityHangzhouChina
  4. 4.State Key Laboratory of Pollution Control and Resource Reuse, School of Environmental Science and EngineeringTongji UniversityShanghaiChina
  5. 5.Basic Science Program, Leidos Biomedical Research, Inc., Cancer and Inflammation ProgramNational Cancer InstituteFrederickUSA
  6. 6.Sackler Institute of Molecular Medicine, Department of Human Genetics and Molecular Medicine Sackler School of MedicineTel Aviv UniversityTel AvivIsrael

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