Preparation and Screening of Catalytic Amyloid Assemblies
Aggregation of proteins into amyloids has long been recognized as one of the major contributors to disease and aging. Amyloids are known to catalyze their own formation but they have been considered the rock-bottom thermodynamic minimum of the protein fold without much functionality. We have recently demonstrated that aggregation of short peptides in the presence of metal ions gives rise to efficient catalytic activity. Here we present a detailed protocol for the synthesis and purification of these peptides and the preparation of amyloid-like fibrils. Then we describe an easy-to-perform, high-throughput assay to measure their hydrolytic activity.
Key wordsSelf-assembly Peptides Amyloids Catalysis Hydrolysis
This work was supported in part by a grant number 1332349 from NSF-EFRI, GM119634 from the NIH, ORAU Ralph E. Powe Junior Faculty Enhancement award and a Humboldt Fellowship to I.V.K. We thank Prof. Olga Makhlynets for constructive suggestions.
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