Abstract
The DNA origami technique is a widely used method to create customized, complex, spatially well-defined two-dimensional (2D) and three-dimensional (3D) DNA nanostructures. These structures have huge potential to serve as smart drug-delivery vehicles and molecular devices in various nanomedical and biotechnological applications. However, so far only little is known about the behavior of these novel structures in living organisms or in cell culture/tissue models. Moreover, enhancing pharmacokinetic bioavailability and transfection properties of such structures still remains a challenge. One intriguing approach to overcome these issues is to coat DNA origami nanostructures with proteins or lipid membranes. Here, we show how cowpea chlorotic mottle virus (CCMV) capsid proteins (CPs) can be used for coating DNA origami nanostructures. We present a method for disassembling native CCMV particles and isolating the pure CP dimers, which can further bind and encapsulate a rectangular DNA origami shape. Owing to the highly programmable nature of DNA origami, packaging of DNA nanostructures into viral protein cages could find imminent uses in enhanced targeting and cellular delivery of various active nano-objects, such as enzymes and drug molecules.
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Acknowledgment
Financial support from the Academy of Finland (grants 263504, 267497, 273645, and 286845), Jane and Aatos Erkko Foundation, Emil Aaltonen Foundation, and Biocentrum Helsinki is gratefully acknowledged. This work was carried out under the Academy of Finland Centers of Excellence Programme (2014–2019) and made use of the Aalto Nanomicroscopy Centre (Aalto NMC) premises.
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Linko, V., Mikkilä, J., Kostiainen, M.A. (2018). Packaging DNA Origami into Viral Protein Cages. In: Wege, C., Lomonossoff, G. (eds) Virus-Derived Nanoparticles for Advanced Technologies. Methods in Molecular Biology, vol 1776. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7808-3_18
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DOI: https://doi.org/10.1007/978-1-4939-7808-3_18
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