Abstract
Tissue transplantation is an important tool for in vivo studies of pattern organization and axis establishment or maintenance in planarians. Further, transplants can inform on cell movements under different regenerative conditions or genetic backgrounds. Here, we detail classical grafting strategies that have been variously employed in planarian research over the last few decades. These protocols remain similarly useful for addressing current and future questions in the field and the step-by-step protocol that we provide allows facile adaptation of this important method into the experimental repertoire of a laboratory.
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Acknowledgments
This work was supported by grant BFU2011-22786 (Ministerio de Economía y Competitividad, Spain) to E.S., and grant 2014 SGR 687 (AGAUR) to E.S. J.I.R-L received a FPI fellowship from the Ministerio de Educación y Ciencia (Spain).
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Video showing the different steps to proceed in the host immobilization (Sarcophagus) (MP4 135851 kb)
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Rojo-Laguna, J.I., Saló, E. (2018). Tissue Transplantations in Planarians. In: Rink, J. (eds) Planarian Regeneration. Methods in Molecular Biology, vol 1774. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7802-1_21
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DOI: https://doi.org/10.1007/978-1-4939-7802-1_21
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7800-7
Online ISBN: 978-1-4939-7802-1
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