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Whole-Mount In Situ Hybridization of Planarians

Part of the Methods in Molecular Biology book series (MIMB,volume 1774)

Abstract

Whole-mount in situ hybridization (WISH) and fluorescent whole-mount in situ hybridization (FISH) allow for visualization of specific mRNA transcripts to answer diverse biological questions. In planarians, in situ hybridization enables determination of gene expression profiles and identification of cell-type specific markers for analyzing experimental treatments. Here, we describe a robust whole-mount protocol for detecting gene expression patterns in the planarian Schmidtea mediterranea.

Key words

  • Whole-mount in situ hybridization (WISH)
  • Fluorescent in situ hybridization (FISH)
  • Planarian
  • Schmidtea mediterranea
  • Riboprobe synthesis
  • Tyramide signal amplification (TSA)

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  • DOI: 10.1007/978-1-4939-7802-1_12
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Acknowledgments

We thank Amanda Adams, Caitlin Dingwall, Melanie Issigonis, and Rachel Roberts-Galbraith for comments on the manuscript and recommendations for processing sexual planarians.

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Correspondence to Ryan S. King .

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King, R.S., Newmark, P.A. (2018). Whole-Mount In Situ Hybridization of Planarians. In: Rink, J. (eds) Planarian Regeneration. Methods in Molecular Biology, vol 1774. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7802-1_12

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  • DOI: https://doi.org/10.1007/978-1-4939-7802-1_12

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7800-7

  • Online ISBN: 978-1-4939-7802-1

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