Whole-mount in situ hybridization (WISH) and fluorescent whole-mount in situ hybridization (FISH) allow for visualization of specific mRNA transcripts to answer diverse biological questions. In planarians, in situ hybridization enables determination of gene expression profiles and identification of cell-type specific markers for analyzing experimental treatments. Here, we describe a robust whole-mount protocol for detecting gene expression patterns in the planarian Schmidtea mediterranea.
- Whole-mount in situ hybridization (WISH)
- Fluorescent in situ hybridization (FISH)
- Riboprobe synthesis
- Tyramide signal amplification (TSA)