Abstract
It is a major challenge in biology to know whether chromosome functions of replication, segregation, gene expression, inheritance, etc. are conserved among evolutionary distant organisms where common structural features are maintained. Establishment of hybrid cell lines between evolutionary distant organisms, such as humans and plants, would be one of the promising synthetic approaches to study the evolutionary conservation of chromosome functions. In this chapter, we describe the protocol for successful establishment of human cell lines with a functional plant chromosome. Systematic analyses of hybrid cells will facilitate the evolutionary study of organisms with respect to chromosome functions. It will also provide a basic platform for genome writing and construction of chromosomal shuttle vectors .
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Acknowledgments
The work was supported by a Grant-in-Aid for JSPS fellows 23-7429 from JSPS KAKENHI.
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Wada, N., Kazuki, Y., Kazuki, K., Inoue, T., Fukui, K., Oshimura, M. (2018). Production of a Human Cell Line with a Plant Chromosome. In: Braman, J. (eds) Synthetic Biology. Methods in Molecular Biology, vol 1772. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7795-6_16
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DOI: https://doi.org/10.1007/978-1-4939-7795-6_16
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-7795-6
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