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Fabrication of Biomolecule Microarrays for Cell Immobilization Using Automated Microcontact Printing

  • Julie Foncy
  • Aurore Estève
  • Amélie Degache
  • Camille Colin
  • Jean Christophe Cau
  • Laurent Malaquin
  • Christophe Vieu
  • Emmanuelle Trévisiol
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1771)

Abstract

Biomolecule microarrays are generally produced by conventional microarrayer, i.e., by contact or inkjet printing. Microcontact printing represents an alternative way of deposition of biomolecules on solid supports but even if various biomolecules have been successfully microcontact printed, the production of biomolecule microarrays in routine by microcontact printing remains a challenging task and needs an effective, fast, robust, and low-cost automation process. Here, we describe the production of biomolecule microarrays composed of extracellular matrix protein for the fabrication of cell microarrays by using an automated microcontact printing device. Large scale cell microarrays can be reproducibly obtained by this method.

Key words

Biomolecule microarrays Cell microarrays Microcontact printing Micropatterning Cell architecture 

Notes

Acknowledgments

This work was supported by the national research agency “LABCOM” program (ANR-13-LAB2-0009-01) and partly supported by LAAS CNRS micro and nanotechnology facilities platform (member of the French RENATECH network). We thank Charline Blatché for her assistance in the cell microarray fabrication.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Julie Foncy
    • 1
  • Aurore Estève
    • 1
  • Amélie Degache
    • 2
  • Camille Colin
    • 1
  • Jean Christophe Cau
    • 2
  • Laurent Malaquin
    • 1
  • Christophe Vieu
    • 1
  • Emmanuelle Trévisiol
    • 1
  1. 1.Laboratory for Analysis and Architecture of Systems (LAAS-CNRS)Université de Toulouse, CNRS, INSAToulouseFrance
  2. 2.INNOPSYSCarbonneFrance

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