Three-Dimensional Cell-Based Microarrays: Printing Pluripotent Stem Cells into 3D Microenvironments

  • Jorge F. Pascoal
  • Tiago G. Fernandes
  • Gregory J. Nierode
  • Maria Margarida Diogo
  • Jonathan S. Dordick
  • Joaquim M. S. Cabral
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1771)

Abstract

Cell-based microarrays are valuable platforms for the study of cytotoxicity and cellular microenvironment because they enable high-throughput screening of large sets of conditions at reduced reagent consumption. However, most of the described microarray technologies have been applied to two-dimensional cultures, which do not accurately emulate the in vivo three-dimensional (3D) cell–cell and cell–extracellular matrix interactions.

Herein, we describe the methodology for production of alginate- and Matrigel-based 3-D cell microarrays for the study of mouse and human pluripotent stem cells on two different chip-based platforms. We further provide protocols for on-chip proliferation/viability analysis and the assessment of protein expression by immunofluorescence.

Key words

3D cell microarray Pluripotent stem cells Cellular microenvironment High-content screening Cytotoxicity 

Notes

Acknowledgments

The work involved in the development of this protocol was funded by NIH (ES020903) and NYSTEM (C026717). JFP acknowledges support from Fundação para a Ciência e Tecnologia (SFRH/BD/79590/2011), which also funded iBB (UID/BIO/04565/2013).

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Jorge F. Pascoal
    • 1
    • 2
  • Tiago G. Fernandes
    • 1
    • 2
  • Gregory J. Nierode
    • 2
  • Maria Margarida Diogo
    • 1
  • Jonathan S. Dordick
    • 2
  • Joaquim M. S. Cabral
    • 1
  1. 1.Department of Bioengineering, Institute for Bioengineering and Biosciences (iBB), Instituto Superior TécnicoUniversidade de LisboaLisbonPortugal
  2. 2.Chemical and Biological Engineering, Center for Biotechnology and Interdisciplinary StudiesRensselaer Polytechnic InstituteTroyUSA

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