3D Plasma Nanotextured® Polymeric Surfaces for Protein or Antibody Arrays, and Biomolecule and Cell Patterning

  • Katerina TsougeniEmail author
  • Kosmas Ellinas
  • George Koukouvinos
  • Panagiota S. Petrou
  • Angeliki Tserepi
  • Sotirios E. Kakabakos
  • Evangelos GogolidesEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1771)


Plasma micro-nanotexturing is a generic technology for topographical and chemical modification of surfaces and their implementation in microfluidics and microarrays. Nanotextured surfaces with desirable chemical functionality (and wetting behavior) have shown excellent biomolecule immobilization and cell adhesion. Specifically, nanotextured hydrophilic areas show (a) strong binding of biomolecules and (b) strong adhesion of cells, while nanotextured superhydrophobic areas show null adsorption of (a) proteins and (b) cells. Here we describe the protocols for (a) biomolecule adsorption control on nanotextured surfaces for microarray fabrication and (b) cell adhesion on such surfaces. 3D plasma nanotextured® substrates are commercialized through Nanoplasmas private company, a spin-off of the National Centre for Scientific Research Demokritos.

Key words

3D plasma micro-nanotexturing Plasma deposition Stable in time desirable chemical functionality Biomolecule arrays Cell patterning 



The following projects are acknowledged for funding of this work: (1) “Love Wave Fully Integrated Lab-on-chip Platform for Food Pathogen Detection”—LOVE FOOD project (Contract No 317742), (2) Horizon 2020-EU 2.1.1, Project ID: 68768, “LOVEFOOD2Market—A portable MicroNanoBioSystem and Instrument for ultra-fast analysis of pathogens in food: Innovation from LOVE-FOOD lab prototype to a pre-commercial instrument” (


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Katerina Tsougeni
    • 1
    • 2
    Email author
  • Kosmas Ellinas
    • 1
    • 2
  • George Koukouvinos
    • 3
  • Panagiota S. Petrou
    • 2
    • 3
  • Angeliki Tserepi
    • 1
    • 2
  • Sotirios E. Kakabakos
    • 2
    • 3
  • Evangelos Gogolides
    • 1
    • 2
    Email author
  1. 1.Institute of Nanoscience and NanotechnologyNCSR DemokritosAttikiGreece
  2. 2.Nanoplasmas PC, A Spin-Out Company of NCSR Demokritos, Lefkippos Technology ParkAttikiGreece
  3. 3.Institute of Nuclear and Radiological Sciences and Technology, Energy and SafetyNCSR DemokritosAttikiGreece

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