RNA-Seq Analysis to Detect Abnormal Fusion Transcripts Linked to Chromothripsis
RNA-Seq approach enables the detection and characterization of fusion or chimeric transcript associated to complex genome rearrangement. Until now, these events are classically identified at DNA level.
Here we describe a complete procedure including a novel way of analyzing reads that combines genomic locations and local coverage to directly infer chimeric junctions with a high sensitivity and specificity, allowing identification of different classes of chimeric RNA events. We also recommend the best practices for the bioinformatics analysis and describe the experimental process for RNA validation using real-time PCR and sequencing.
Key wordsRNA-Seq Chimeras Genomic rearrangement Chromothripsis Transcription Chimeric RNAs Fusion genes NGS