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Chromothripsis pp 183-195 | Cite as

Generation of Micronuclei and Detection of Chromosome Pulverization

  • Monique N. H. Luijten
  • Jeannie X.  T. Lee
  • Sixun Chen
  • Karen C. Crasta
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1769)

Abstract

Lagging chromosomes that arise after chromosome mis-segregation during cell division can be encapsulated within small structures known as micronuclei. A link between whole-chromosome mis-segregation and chromothripsis has been demonstrated via micronuclear chromosome pulverization. Here, we describe methods to efficiently generate micronuclei and examine downstream cell fates, specifically with regard to DNA damage and chromosome pulverization.

Key words

Micronuclei Chromosome mis-segregation Aneuploidy Mitosis DNA breaks Chromothripsis 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Monique N. H. Luijten
    • 1
  • Jeannie X.  T. Lee
    • 1
  • Sixun Chen
    • 1
    • 2
  • Karen C. Crasta
    • 1
    • 2
    • 3
    • 4
  1. 1.Lee Kong Chian School of Medicine, Nanyang Technological UniversitySingaporeSingapore
  2. 2.A*STAR Institute of Molecular and Cell BiologySingaporeSingapore
  3. 3.School of Biological Sciences, Nanyang Technological UniversitySingaporeSingapore
  4. 4.Department of MedicineImperial College LondonLondonUK

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