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The Use of Laser Microdissection to Investigate Cell-Specific Gene Expression in Orchid Tissues

  • Raffaella Balestrini
  • Valeria Fochi
  • Alessandro Lopa
  • Silvia Perotto
Protocol
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

In the past decade, laser microdissection (LMD) technology has been widely applied to plant tissues, highlighting the role of different cell-type populations during plant–microbe interactions. In this chapter, a method to apply the LMD approach to study gene expression in specific cell-type populations of orchid mycorrhizal protocorms and roots is described in detail, starting from the preparation of biological material to gene expression analysis by RT-PCR.

Key words

Laser microdissection Gene expression Orchid Serapias vomeracea Tulasnella calospora 

Notes

Acknowledgments

The LMD system upgrade was possible thanks to the financial support of the “Compagnia di San Paolo” (Torino, Italy).

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Raffaella Balestrini
    • 1
  • Valeria Fochi
    • 2
  • Alessandro Lopa
    • 2
  • Silvia Perotto
    • 2
    • 1
  1. 1.Istituto per la Protezione Sostenibile delle Piante, Consiglio Nazionale delle Ricerche (CNR)TurinItaly
  2. 2.Dipartimento di Scienze della Vita e Biologia dei SistemiUniversità degli Studi di TorinoTurinItaly

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