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Genetic Transformation on Orchid Species: An Overview of Approaches and Methodologies

  • Masahiro Mii
  • Dong Poh Chin
Protocol
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

Genetic transformation technologies have been used to overcome the difficulty in achieving the breeding objectives by conventional methods. Although orchids have been rather difficult targets for genetic transformation, it is now feasible to transform some of the commercially important orchid species due to the progress made in technologies for overcoming some difficulties during the past two decades. In this chapter, we overview the past efforts in establishing genetic transformation methods on orchids and also summarize the important factors affecting successful transformation.

Key words

Acetosyringone Agrobacterium tumefaciens Biolistic method Beta-glucuronidase (GUS) gene Carbenicillin Cefotaxime Co-cultivation Embryogenic callus Foreign gene Gene isolation Gene transfer Genetic transformation Green fluorescent protein (GFPHygromycin Kanamycin Meropenem Particle bombardment Plant regeneration Plasmid Protocorm Protocorm-like body (PLB) Phosphinothricin (PPT) Ti plasmid T-DNA 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Center for Environment, Health and Field SciencesChiba UniversityKashiwaJapan

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