CpG Islands pp 83-107 | Cite as

Infinium DNA Methylation Microarrays on Formalin-Fixed, Paraffin-Embedded Samples

  • Sebastian Moran
  • Manel EstellerEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1766)


Formalin-fixed, paraffin-embedded (FFPE) samples usually yield fragmented DNA, which is incompatible with traditional Infinium Methylation beadchips. In this chapter, we aim to explain in detail all the processes carried out in order to obtain high-quality methylation profiles from FFPE samples through an FFPE restoration procedure. High-throughput methylation profiling platforms such as the Infinium Methylation beadchips have been extensively used by the scientific community to elucidate many hypotheses in multiple scenarios, now with the incorporation of FFPE samples to these platforms, a myriad of new opportunities is being opened.

Key words

Epigenetics DNA methylation Formalin-fixed, paraffin-embedded FFPE Methylation Microarray Restoration Degraded DNA 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Cancer Epigenetics and Biology Program (PEBC)Bellvitge Biomedical Research Institute (IDIBELL)BarcelonaSpain
  2. 2.Department of Physiological Sciences II, School of MedicineUniversity of BarcelonaBarcelonaSpain
  3. 3.Institució Catalana de Recerca i Estudis Avançats (ICREA)BarcelonaSpain

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