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CpG Islands pp 231-238 | Cite as

Genome-Wide Mapping of Protein–DNA Interactions on Nascent Chromatin

  • Chenhuan Xu
  • Victor G. Corces
Part of the Methods in Molecular Biology book series (MIMB, volume 1766)

Abstract

Chromatin immunoprecipitation (ChIP) is the most widely used method to analyze protein–DNA interactions in vivo. Coupled with next generation sequencing, ChIP-seq experiments map protein–DNA interactions in a genome-wide fashion. Here we describe a novel method called nasChIP-seq for mapping genome-wide occupancy of posttranslationally modified histones or transcription factors on newly replicated DNA.

Key words

Chromatin immunoprecipitation ChIP-seq DNA replication Nascent chromatin Click chemistry 

Notes

Acknowledgments

Work in the authors’ laboratory was supported by U.S. Public Health Service Award R01 GM035463 from the National Institutes of Health. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Chenhuan Xu
    • 1
  • Victor G. Corces
    • 1
  1. 1.Department of BiologyEmory UniversityAtlantaUSA

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