Molecular Analysis of the Microbiome in Colorectal Cancer
The human gut microbiota plays a major role in the development of colorectal cancer (CRC). Many studies have attempted to define links between microbiota residents, their function and disease development. We now have incredible molecular tools to allow us to study the gut microbiome however in order to make best use of these sophisticated approaches we need to ensure that samples are collected and processed using standardized and reproducible protocols. Here we provide an overview of molecular analysis methods and describe protocols for collecting and processing clinical samples for subsequent microbiome analysis.
Key wordsColorectal cancer Microbiome Microbial diversity Clinical samples Stool Biopsy DNA RNA Bacteria Fungi Molecular microbiology
This work was supported by grants from The Chief Scientist Office, Friends of Anchor, NHS Grampian Endowments, NHS Grampian Gastroenterology Endowment funds as well as donations from CRC patients.
- 19.Rajilić-Stojanović M, Heilig HG, Molenaar D et al (2009) Development and application of the human intestinal tract chip, a phylogenetic microarray: analysis of universally conserved phylotypes in the abundant microbiota of young and elderly adults. Environ Microbiol 11(7):1736–1751CrossRefPubMedPubMedCentralGoogle Scholar