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Testing Cell-Based Immunotherapy for Colorectal Cancer

  • Jong Soon Kang
  • Yeo Dae Yoon
  • Myeong Youl Lee
  • Chang Woo Lee
  • Suk Jong Lee
  • Sang-Bae Han
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1765)

Abstract

Cell-based immunotherapy for cancer is emerging as an attractive alternative to conventional small-molecule or antibody-based treatment. Due to the characteristics of cell-based therapy, validation of test materials before in vivo administration is required. Here we describe general validation steps for preclinical evaluation of cell-based immunotherapy. We also describe a xenograft model of human colorectal cancer. This model can be used for applied to preclinical evaluation of various cell-based therapy regimens for colorectal cancer treatment.

Key words

Immunotherapy Immune cells Colorectal cancer Cell line Human tumor xenograft model 

Notes

Acknowledgment

This work was supported by grants funded by the KRIBB Research Initiative Program and the Korean Government (NRF-2008-0062275).

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Jong Soon Kang
    • 1
  • Yeo Dae Yoon
    • 1
  • Myeong Youl Lee
    • 1
  • Chang Woo Lee
    • 1
  • Suk Jong Lee
    • 2
  • Sang-Bae Han
    • 2
  1. 1.Korea Research Institute of Bioscience and BiotechnologyCheongjuRepublic of Korea
  2. 2.College of PharmacyChungbuk National UniversityCheongjuRepublic of Korea

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