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Colorectal Cancer Detection Using Targeted LC-MS Metabolic Profiling

  • Danijel Djukovic
  • Jiangjiang Zhang
  • Daniel Raftery
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1765)

Abstract

Colorectal cancer (CRC) is one of the most commonly diagnosed malignancies and causes of cancer death. While the mortality rates from CRC have steadily declined, 50,000 individuals in the USA (and many times this number worldwide) still succumb to this illness every year. Early detection of CRC is the most critical need for improving 5-year survival and cure rates. Currently available CRC diagnostic techniques often miss early stage disease such that only 40% of newly diagnosed CRC patients are treated for local disease, Therefore, development of new screening methods that are highly sensitive, specific, noninvasive and easily accessible are critically desired for the early diagnosis and significant reduction in death rate from CRC. In this chapter we describe a targeted LC-MS based metabolic profiling approach used for the discovery of CRC metabolite biomarker candidates, based on highly reproducible hydrophilic interaction liquid chromatography coupled to triple-quadrupole mass spectrometry (HILIC-LC-QQQ-MS). A partial least squares-discriminant analysis (PLS-DA) model was able to differentiate CRC patients from both healthy controls and polyp patients, as well as to distinguish CRC patients based on the cancer stage.

Key words

Colorectal cancer (CRC) Polyps Metabolomics Targeted metabolic profiling LC-MS Metabolite biomarkers 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Danijel Djukovic
    • 1
  • Jiangjiang Zhang
    • 1
  • Daniel Raftery
    • 1
  1. 1.Department of Anesthesiology and Pain MedicineUniversity of WashingtonSeattleUSA

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