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Correlative 3D Structured Illumination Microscopy and Single-Molecule Localization Microscopy for Imaging Cancer Invasion

Part of the Methods in Molecular Biology book series (MIMB,volume 1764)

Abstract

Super-resolution microscopy methods enable resolution of biological molecules in their cellular or tissue context at the nanoscale. Different methods have their strengths and weaknesses. Here we present a method that enables correlative confocal, structured illumination microscopy (SIM) and single-molecule localization microscopy (SMLM) imaging of structures involved in formation of invadopodia on the same sample. This enables up to four colors to be visualized in three dimensions at a resolution of between 120 and 10 nm for SIM and SMLM, respectively.

Key words

  • Invasion
  • Microscopy
  • Super-resolution
  • Cells

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Correspondence to Ann P. Wheeler .

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Pinnington, S.J.L., Marshall, J.F., Wheeler, A.P. (2018). Correlative 3D Structured Illumination Microscopy and Single-Molecule Localization Microscopy for Imaging Cancer Invasion. In: Marsh, J. (eds) Protein Complex Assembly. Methods in Molecular Biology, vol 1764. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7759-8_15

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  • DOI: https://doi.org/10.1007/978-1-4939-7759-8_15

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