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Global Characterization of Protein Complexes by Biochemical Purification-Mass Spectrometry (BP/MS)

  • Reza Pourhaghighi
  • Andrew Emili
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1764)

Abstract

A proteomic platform for global analysis of protein complexes and protein-protein interactions (PPIs) is described. Briefly, after comprehensive physiochemical separation of soluble protein extracts using non-denaturing ion exchange chromatography (IEX), each fraction is subjected to quantitative tandem mass spectrometry analysis.

Key words

Protein-Protein Interaction High Performance Liquid Chromatography (HPLC) Intact Protein Separation Ion Exchange Chromatography (IEX) Nano-Liquid Chromatography Tandem Mass Spectrometry (nLC-MS/MS) 

Reference

  1. 1.
    Havugimana PC, Hart GT, Nepusz T et al (2012) A census of human soluble protein complexes. Cell 150(5):1068–1081CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Donnelly Centre for Cellular and Biomolecular ResearchUniversity of TorontoTorontoCanada
  2. 2.Departments of Biology and BiochemistryBoston UniversityTorontoCanada

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