Immunoprecipitation of Membrane Proteins from Arabidopsis thaliana Root Tissue
Here, we present different methods for immunoprecipitating membrane proteins of Arabidopsis thaliana root material. We describe two extraction methods for the precipitation either for an integral membrane protein of the endoplasmic reticulum (ER) or a peripheral membrane protein partially localized at the plasma membrane, where we precipitate the protein out of the total membrane as well as total cytosolic fractions.
Key wordsImmunoprecipitation Integral membrane proteins Peripheral membrane proteins Membrane fraction Cytosolic fraction Cross-linked beads Arabidopsis thaliana
Establishment for this protocol was supported by a grant from the Austrian science fund FWF (P 29754 to SW, JK-V; V382 to BK), the Austrian Academy of Science to (LD-A (DOC-ÖAW 24195), the Hochschuljubiläumsstiftung der Stadt Wien (H-235701/2014 to BK), the Vienna Science and Technology Fund (WWTF) (to JK-V), and the European Research Council (ERC) (Starting Grant 639478-AuxinER to JK-V).
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