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Immunoprecipitation of Membrane Proteins from Arabidopsis thaliana Root Tissue

  • Sascha Waidmann
  • Lucinda De-Araujo
  • Jürgen Kleine-Vehn
  • Barbara Korbei
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1761)

Abstract

Here, we present different methods for immunoprecipitating membrane proteins of Arabidopsis thaliana root material. We describe two extraction methods for the precipitation either for an integral membrane protein of the endoplasmic reticulum (ER) or a peripheral membrane protein partially localized at the plasma membrane, where we precipitate the protein out of the total membrane as well as total cytosolic fractions.

Key words

Immunoprecipitation Integral membrane proteins Peripheral membrane proteins Membrane fraction Cytosolic fraction Cross-linked beads Arabidopsis thaliana 

Notes

Acknowledgments

Establishment for this protocol was supported by a grant from the Austrian science fund FWF (P 29754 to SW, JK-V; V382 to BK), the Austrian Academy of Science to (LD-A (DOC-ÖAW 24195), the Hochschuljubiläumsstiftung der Stadt Wien (H-235701/2014 to BK), the Vienna Science and Technology Fund (WWTF) (to JK-V), and the European Research Council (ERC) (Starting Grant 639478-AuxinER to JK-V).

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Copyright information

© Springer Science+Business Media, LLC 2018

Authors and Affiliations

  • Sascha Waidmann
    • 1
  • Lucinda De-Araujo
    • 1
  • Jürgen Kleine-Vehn
    • 1
  • Barbara Korbei
    • 1
  1. 1.Department of Applied Genetics and Cell Biology (DAGZ)University of Natural Resources and Life Sciences (BOKU)ViennaAustria

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