Abstract
Light sheet fluorescence microscopy (LSFM) allows sustained and repeated optical sectioning of living specimens at high spatial and temporal resolution, with minimal photodamage. Here, we describe in detail both the hardware and the software elements of a live imaging method based on LSFM and optimized for tracking and 3D scanning of Arabidopsis root tips grown vertically in physiological conditions. The system is relatively inexpensive and with minimal footprint; hence it is well suited for laboratories of any size.
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This work was supported by BBSRC grant BB/M002624/1.
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Baesso, P., Randall, R.S., Sena, G. (2018). Light Sheet Fluorescence Microscopy Optimized for Long-Term Imaging of Arabidopsis Root Development. In: Ristova, D., Barbez, E. (eds) Root Development. Methods in Molecular Biology, vol 1761. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7747-5_11
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DOI: https://doi.org/10.1007/978-1-4939-7747-5_11
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