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Light Sheet Fluorescence Microscopy Optimized for Long-Term Imaging of Arabidopsis Root Development

  • Paolo Baesso
  • Ricardo S. Randall
  • Giovanni Sena
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1761)

Abstract

Light sheet fluorescence microscopy (LSFM) allows sustained and repeated optical sectioning of living specimens at high spatial and temporal resolution, with minimal photodamage. Here, we describe in detail both the hardware and the software elements of a live imaging method based on LSFM and optimized for tracking and 3D scanning of Arabidopsis root tips grown vertically in physiological conditions. The system is relatively inexpensive and with minimal footprint; hence it is well suited for laboratories of any size.

Key words

Microscopy Live imaging Morphodynamics Arabidopsis root Tracking Control software 

Notes

Acknowledgments

This work was supported by BBSRC grant BB/M002624/1.

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Copyright information

© Springer Science+Business Media, LLC 2018

Authors and Affiliations

  • Paolo Baesso
    • 1
  • Ricardo S. Randall
    • 1
  • Giovanni Sena
    • 1
  1. 1.Department of Life SciencesImperial College LondonLondonUK

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