Diagnosis and Pathological Analysis of Patient Cancers by Detection of Proliferating Cells in Gelfoam® Histoculture
Patient tumors grew in Gelfoam® histoculture with maintenance of tissue architecture, tumor-stromal interaction, and differentiated functions. In this chapter, we review the use of Gelfoam® histoculture to demonstrate proliferation indices of major solid cancer types explanted directly from surgery. Cell proliferation was visualized by histological autoradiography within the cultured tissues after [3H]thymidine incorporation by the proliferating cells. Epilumination polarization microscopy enables high-resolution imaging of the autoradiography of each cell. The histological status of the cultured tissues can be assessed simultaneously with the proliferation status. Carcinomas were observed to have areas of high epithelial proliferation with quiescent stromal cells. Sarcomas have high proliferation of the cancer cells of mesenchymal organ. Normal tissues can also proliferate at high rates. Mean growth fraction index (GFI) was highest for patient tumors with the pure subtype of small-cell lung cancer than other types of lung cancer.
Key wordsGelfoam® Three-dimensional histoculture Tumors Lung cancer Small cell Adenocarcinoma Squamous-cell carcinoma Autoradiography Polarization Microscopy Proliferation index
- 3.Minna JD, Higgins GA, Glatstein EJ (1981) Cancer of the lung. In: DeVita VT, Hellman S, Rosenberg SA (eds) Principles and Practice of Oncology. J. B. Lippincott Co., Philadelphia, pp 396–474Google Scholar
- 5.Feinstem AR, Gelfman NA, Yesner R, Auerbach O, Hackel DB, Pratt PC (1970) Observer variability in the histopathologic diagnosis of lung cancer. Am Rev Respir Dis 101:671–684Google Scholar
- 9.Yano S, Miwa S, Mii S, Hiroshima Y, Uehara F, Yamamoto M, Kishimoto H, Tazawa H, Bouvet M, Fujiwara T, Hoffman RM (2014) Invading cancer cells are predominantly in G0/G1 resulting in chemoresistance demonstrated by real-time FUCCI imaging. Cell Cycle 13:953–960Google Scholar