Expression and Targeting of Tumor Markers in Gelfoam® Histoculture: Potential Individualized Assays for Immuno-Oncology

  • Robert M. Hoffman
  • Fiorella Guadagni
Part of the Methods in Molecular Biology book series (MIMB, volume 1760)


Tumor-specific antigens are important in the study of tumor biology, tumor diagnosis, and prognosis and as targets for tumor therapy. This chapter reviews patient colon, breast, and ovarian tumors in 3-dimensional Gelfoam® histoculture maintaining in vivo-like expression of the important tumor antigens, for example TAG-72 and CEA. We have also reviewed that fluorescent antibodies can target tumors in Gelfoam® histoculture, thereby providing an assay for individual patients for sensitivity to therapeutic antibodies which have become so important in immuno-oncology and other cancer therapies.

Key words

Histoculture Gelfoam Tumor antigens Expression Monoclonal antibodies Targeting Immuno-oncology 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.AntiCancer, Inc.San DiegoUSA
  2. 2.Department of SurgeryUCSDSan DiegoUSA
  3. 3.San Raffaele Roma Open UniversityRomeItaly
  4. 4.IRCCS San Raffaele PisanaRomeItaly

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