In Vivo-Like Growth Patterns of Multiple Types of Tumors in Gelfoam® Histoculture

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1760)

Abstract

Diverse human tumors obtained directly from surgery or biopsy can grow at high frequency in 3-dimensional Gelfoam® histoculture for long periods of time and still maintain many of their in vivo properties. The in vivo properties maintained in vitro include 3-dimensional growth; maintenance of tissue organization and structure, such as changes associated with oncogenic transformation; retention of differentiated function; tumorigenicity; and growth of multiple types of cells from a single tumor.

Key words

Gelfoam® histoculture Tumors 3-Dimensional Tissue architecture function 

References

  1. 1.
    Fraslin J, Kneip B, Vaulont S, Glaise D, Munnich A, Guguen-Guillouzo C (1985) Dependence of hepatocyte-specific gene expression on cell-cell interactions in primary culture. EMBO J 4:2487–2491PubMedPubMedCentralGoogle Scholar
  2. 2.
    Clayton DF, Darnell JE Jr (1983) Changes in liver-specific compared to common gene transcription during primary culture of mouse hepatocytes. Mol Cell Biol 3:1552–1561CrossRefPubMedPubMedCentralGoogle Scholar
  3. 3.
    Lawler EM, Miller FR, Heppner GH (1983) Significance of three-dimensional growth patterns of mammary tissues in collagen gels. In Vitro 19:600–610CrossRefPubMedGoogle Scholar
  4. 4.
    Leighton J, Justh G, Esper M, Kronenthal RL (1967) Collagen-coated cellulose sponge: three dimensional matrix for tissue culture of Walker tumor 256. Science 155:1259–1261CrossRefPubMedGoogle Scholar
  5. 5.
    Yang J, Richards J, Bowman P, Guzman R, Enami J, McCormick K, Hamamoto S, Pitelka D, Nandi S (1979) Sustained growth and three-dimensional organization of primary mammary tumor epithelial cells embedded in collagen gels. Proc Natl Acad Sci U S A 76:3401–3405CrossRefPubMedPubMedCentralGoogle Scholar
  6. 6.
    Miller BE, Miller FR, Heppner GH (1985) Factors affecting growth and drug sensitivity of mouse mammary tumor lines in collagen gel cultures. Cancer Res 45:4200–4205PubMedGoogle Scholar
  7. 7.
    Miller BE, Miller FR, Heppner GH (1981) Interactions between tumor subpopulations affecting their sensitivity to the antineoplastic agents cyclophosphamide and methotrexate. Cancer Res 41:4378–4381PubMedGoogle Scholar
  8. 8.
    Freeman AE, Hoffman RA (1986) In vivo-like growth of human tumors in vitro. Proc Natl Acad Sci U S A 83:2694–2698CrossRefPubMedPubMedCentralGoogle Scholar
  9. 9.
    Vescio RA, Redfern CA, Nelson TJ, Ugoretz S, Stern PH, Hoffman RM (1987) In vivo-like drug responses of human tumors growing in three-dimensional gel-supported primary culture. Proc Natl Acad Sci U S A 84:5029–5033CrossRefPubMedPubMedCentralGoogle Scholar
  10. 10.
    Bhatia S, Frangioni JV, Hoffman RM, Iafrate AJ, Polyak K (2012) The challenges posed by cancer heterogeneity. Nat Biotechnol 30:604–610CrossRefPubMedGoogle Scholar
  11. 11.
    Lockshin A, Giovanella B, DeIpolyi PD, Williams LJ, Mendoza JT, Yim SO, Stehlin JS (1985) Exceptional lethality for nude mice of cells derived from a primary human melanoma. Cancer Res 45:345–350PubMedGoogle Scholar
  12. 12.
    Hoffman RM (2010) Histocultures and theiruse. In: Encyclopedia of life sciences. Wiley,Chichester.  https://doi.org/10.1002/9780470015902.a0002573.pub2
  13. 13.
    Hoffman RM (2013) Tissue culture. In: Brenner’s encyclopedia of genetics, 2nd edn, vol. 7. Elsevier, pp. 73–76.Google Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.AntiCancer, Inc.San DiegoUSA
  2. 2.Department of SurgeryUniversity of CaliforniaSan DiegoUSA

Personalised recommendations