In Vivo-Like Growth Patterns of Multiple Types of Tumors in Gelfoam® Histoculture

  • Robert M. Hoffman
  • Aaron E. Freeman
Part of the Methods in Molecular Biology book series (MIMB, volume 1760)


Diverse human tumors obtained directly from surgery or biopsy can grow at high frequency in 3-dimensional Gelfoam® histoculture for long periods of time and still maintain many of their in vivo properties. The in vivo properties maintained in vitro include 3-dimensional growth; maintenance of tissue organization and structure, such as changes associated with oncogenic transformation; retention of differentiated function; tumorigenicity; and growth of multiple types of cells from a single tumor.

Key words

Gelfoam® histoculture Tumors 3-Dimensional Tissue architecture function 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.AntiCancer, Inc.San DiegoUSA
  2. 2.Department of SurgeryUniversity of CaliforniaSan DiegoUSA

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