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Comparison of “Dimensionality” of Cancer Cell Culture in Gelfoam® Histoculture and Matrigel

  • Yasunori Tome
  • Fuminari Uehara
  • Fuminori Kanaya
  • Robert M. Hoffman
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1760)

Abstract

Cell and tissue culture can be performed on different substrates such as on plastic, in Matrigel™, and on Gelfoam®, a sponge matrix. Each of these substrates consists of a very different surface, ranging from hard and inflexible, a gel, and a sponge-matrix, respectively. Folkman and Moscona found that cell shape was tightly coupled to proper gene expression. The flexibility of a substrate is important for cells to maintain their optimal shape. Human osteosarcoma cells, stably expressing a fusion protein of av integrin, and green fluorescent protein (GFP), grew as a simple monolayer without any structure formation on the surface of a plastic dish. When the osteosarcoma cells were cultured within Matrigel, the cancer cells formed colonies but no other structures. When the cancer cells were seeded on Gelfoam®, the cells formed 3-dimensional tissue-like structures. These results indicate that Gelfoam® histoculture, unlike Matrigel™ culture, is true 3-dimensional.

Key words

Cancer cells Monolayer Matrigel™ Gelfoam® Green fluorescent protein (GFP) Fluorescence imaging Rendering Dimensionality Aggregation Tissue Structures 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Yasunori Tome
    • 1
    • 2
    • 3
  • Fuminari Uehara
    • 1
    • 2
    • 3
  • Fuminori Kanaya
    • 3
  • Robert M. Hoffman
    • 1
    • 4
  1. 1.AntiCancer Inc.San DiegoUSA
  2. 2.Department of SurgeryUniversity of CaliforniaSan DiegoUSA
  3. 3.Department of Orthopedic SurgeryGraduate School of Medicine, University of the RyukyusOkinawaJapan
  4. 4.Department of SurgeryUCSDSan DiegoUSA

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