Comparison of “Dimensionality” of Cancer Cell Culture in Gelfoam® Histoculture and Matrigel
Cell and tissue culture can be performed on different substrates such as on plastic, in Matrigel™, and on Gelfoam®, a sponge matrix. Each of these substrates consists of a very different surface, ranging from hard and inflexible, a gel, and a sponge-matrix, respectively. Folkman and Moscona found that cell shape was tightly coupled to proper gene expression. The flexibility of a substrate is important for cells to maintain their optimal shape. Human osteosarcoma cells, stably expressing a fusion protein of av integrin, and green fluorescent protein (GFP), grew as a simple monolayer without any structure formation on the surface of a plastic dish. When the osteosarcoma cells were cultured within Matrigel, the cancer cells formed colonies but no other structures. When the cancer cells were seeded on Gelfoam®, the cells formed 3-dimensional tissue-like structures. These results indicate that Gelfoam® histoculture, unlike Matrigel™ culture, is true 3-dimensional.
Key wordsCancer cells Monolayer Matrigel™ Gelfoam® Green fluorescent protein (GFP) Fluorescence imaging Rendering Dimensionality Aggregation Tissue Structures
- 10.Leighton J, Kline I, Belkin M, Tetenbaum Z (1956) Studies on human cancer using sponge-matrix tissue culture. III. The invasive properties of a carcinoma (strain HeLa) as influenced by temperature variations, by conditioned media, and in contact with rapidly growing chick embryonic tissue. J Natl Cancer Inst 16:1353–1373PubMedGoogle Scholar
- 14.Leighton J (1954) Studies on human cancer using sponge matrix tissue culture. I. The growth patterns of a malignant melanoma, adenocarcinoma of the parotid gland, papillary adenocarcinoma of the thyroid gland, adenocarcinoma of the pancreas, and epidermoid carcinoma of the uterine cervix (Gey's HeLa strain). Tex Rep Biol Med 12:847–864PubMedGoogle Scholar
- 27.Kubota T, Sasano N, Abe O, Nakao I, Kawamura E, Saito T, Endo M, Kimura K, Demura H, Sasano H, Nagura H, Ogawa N, Hoffman RM (1995) The chemosensitivity study group for the histoculture drug-response assay. Potential of the histoculture drug response assay to contribute to cancer patient survival. Clin Cancer Res 1:1537–1543PubMedGoogle Scholar