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3D Sponge-Matrix Histoculture pp 163–186Cite as

Nerve Growth and Interaction in Gelfoam® Histoculture: A Nervous System Organoid

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Part of the Methods in Molecular Biology book series (MIMB,volume 1760)

Abstract

Nestin-expressing hair follicle-associated pluripotent (HAP) stem cells reside mainly in the bulge area (BA) of the hair follicle but also in the dermal papilla (DP). The BA appears to be origin of HAP stem cells. Long-term Gelfoam® histoculture was established of whiskers isolated from transgenic mice, in which there is nestin-driven green fluorescent protein (ND-GFP). HAP stem cells trafficked from the BA toward the DP area and extensively grew out onto Gelfoam® forming nerve-like structures. These fibers express the neuron marker β-III tubulin-positive fibers and consisted of ND-GFP-expressing cells and extended up to 500 mm from the whisker nerve stump in Gelfoam® histoculture. The growing fibers had growth cones on their tips expressing F-actin indicating that the fibers were growing axons. HAP stem cell proliferation resulted in elongation of the follicle nerve and interaction with other nerves in 3D Gelfoam® histoculture, including the sciatic nerve, trigeminal nerve, and trigeminal nerve ganglion.

Key words

  • Nestin, Green fluorescent protein
  • Hair follicle
  • Stem cells
  • Sensory nerve
  • Sciatic nerve
  • Trigeminal nerve
  • Nerve interaction
  • Gelfoam® histoculture

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  • DOI: 10.1007/978-1-4939-7745-1_16
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References

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Author information

Authors and Affiliations

  1. AntiCancer Inc., San Diego, CA, USA

    Robert M. Hoffman, Sumiyuki Mii & Jennifer Duong

  2. Department of Surgery, UCSD, San Diego, CA, USA

    Robert M. Hoffman

  3. Department of Dermatology, Kitasato University School of Medicine, Minami Ward, Sagihara, Japan

    Sumiyuki Mii & Yasuyuki Amoh

Authors
  1. Robert M. Hoffman
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  2. Sumiyuki Mii
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  3. Jennifer Duong
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  4. Yasuyuki Amoh
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Corresponding author

Correspondence to Robert M. Hoffman .

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Editors and Affiliations

  1. AntiCancer, Inc., San Diego, California, USA

    Dr. Robert M. Hoffman

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Hoffman, R.M., Mii, S., Duong, J., Amoh, Y. (2018). Nerve Growth and Interaction in Gelfoam® Histoculture: A Nervous System Organoid. In: Hoffman, R. (eds) 3D Sponge-Matrix Histoculture. Methods in Molecular Biology, vol 1760. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7745-1_16

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  • DOI: https://doi.org/10.1007/978-1-4939-7745-1_16

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  • Publisher Name: Humana Press, New York, NY

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