In Vivo-Like Cell-Cycle Phase Distribution of Cancer Cells in Gelfoam® Histoculture Observed in Real Time by FUCCI Imaging

  • Robert M. Hoffman
  • Shuya Yano
Part of the Methods in Molecular Biology book series (MIMB, volume 1760)


FUCCI color codes cells as they express different color fluorescent proteins as they go through phases of the cell cycle. The cell cycle phase distribution of cancer cells in Gelfoam® histoculture was similar to in vivo tumors, whereby only the surface cells proliferate and interior cells are quiescent in G0/G1. In contrast, in 2D cancer-cell culture, most of the cells are always cycling. The cancer cells responded similarly to toxic chemotherapy in Gelfoam® culture as in vivo; as such, therapy is cell cycle dependent. In 2D culture, cancer cells were much more chemosensitive. These results indicate why the drug response pattern of tumors in Gelfoam® histoculture reflects what is observed in vivo.

Key words

FUCCI (fluorescence ubiquitination cell cycle indicator) Color-coding Gelfoam® histoculture 3D culture Imaging Cell cycle Chemotherapy 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.AntiCancer Inc.San DiegoUSA
  2. 2.Department of SurgeryUniversity of CaliforniaSan DiegoUSA
  3. 3.Department of Gastroenterological SurgeryGraduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama UniversityOkayamaJapan

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