In Vivo-Like Cell-Cycle Phase Distribution of Cancer Cells in Gelfoam® Histoculture Observed in Real Time by FUCCI Imaging
FUCCI color codes cells as they express different color fluorescent proteins as they go through phases of the cell cycle. The cell cycle phase distribution of cancer cells in Gelfoam® histoculture was similar to in vivo tumors, whereby only the surface cells proliferate and interior cells are quiescent in G0/G1. In contrast, in 2D cancer-cell culture, most of the cells are always cycling. The cancer cells responded similarly to toxic chemotherapy in Gelfoam® culture as in vivo; as such, therapy is cell cycle dependent. In 2D culture, cancer cells were much more chemosensitive. These results indicate why the drug response pattern of tumors in Gelfoam® histoculture reflects what is observed in vivo.
Key wordsFUCCI (fluorescence ubiquitination cell cycle indicator) Color-coding Gelfoam® histoculture 3D culture Imaging Cell cycle Chemotherapy
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