In Memoriam: Joseph Leighton, 1921–1999—Father of 3-Dimensional Tissue Culture
Joseph Leighton developed sponge-matrix histoculture in the early 1950s and is the father of true 3D cell and tissue culture. Dr. Leighton demonstrated that sponge-matrix histoculture could replicate in vivo-like tissue structure and function by allowing cells to take their natural shape.
Key wordsSponge-matrix 3-Dimensional Histoculture Tumors Cell-self-organization Organoid Tissue
- 5.Leighton J, Kline I, Belkin M, Tetenbaum Z (1956) Studies on human cancer using sponge-matrix tissue culture. III. The invasive properties of a carcinoma (strain HeLa) as influenced by temperature variations, by conditioned media, and in contact with rapidly growing chick embryonic tissue. J Natl Cancer Inst 16:1353–1373PubMedGoogle Scholar