In Memoriam: Joseph Leighton, 1921–1999—Father of 3-Dimensional Tissue Culture

  • Robert M. Hoffman
Part of the Methods in Molecular Biology book series (MIMB, volume 1760)


Joseph Leighton developed sponge-matrix histoculture in the early 1950s and is the father of true 3D cell and tissue culture. Dr. Leighton demonstrated that sponge-matrix histoculture could replicate in vivo-like tissue structure and function by allowing cells to take their natural shape.

Key words

Sponge-matrix 3-Dimensional Histoculture Tumors Cell-self-organization Organoid Tissue 


  1. 1.
    Schaeffer WI (1999) In memorium—a tribute to Dr. Joseph Leighton. Methods Cell Sci 21:v–viCrossRefGoogle Scholar
  2. 2.
    Leighton J (1951) A sponge matrix method for tissue culture; formation of organized aggregates of cells in vitro. J Natl Cancer Inst 12:545–561PubMedGoogle Scholar
  3. 3.
    Leighton J (1959) Aggregate replication, a factor in the growth of cancer. Science 129:466–467CrossRefPubMedGoogle Scholar
  4. 4.
    Leighton J (1954) The growth patterns of some transplantable animal tumors in sponge matrix tissue culture. J Natl Cancer Inst 15:275–293PubMedGoogle Scholar
  5. 5.
    Leighton J, Kline I, Belkin M, Tetenbaum Z (1956) Studies on human cancer using sponge-matrix tissue culture. III. The invasive properties of a carcinoma (strain HeLa) as influenced by temperature variations, by conditioned media, and in contact with rapidly growing chick embryonic tissue. J Natl Cancer Inst 16:1353–1373PubMedGoogle Scholar
  6. 6.
    Leighton J, Kline I (1954) Studies on human cancer using sponge matrix tissue culture. II. Invasion of connective tissue by carcinoma (strain HeLa). Tex Rep Biol Med 12:865–873PubMedGoogle Scholar
  7. 7.
    Leighton J, Kalla R, Kline I, Belkin M (1959) Pathogenesis of tumor invasion. I. Interaction between normal tissues and transformed cells in tissue culture. Cancer Res 19:23–27PubMedGoogle Scholar
  8. 8.
    Leighton J, Kline I, Hc O (1956) Transformation of normal human fibroblasts into histologically malignant tissue in vitro. Science 123:502–503CrossRefPubMedGoogle Scholar
  9. 9.
    Leighton J, Kline I, Belkin M, Hc O (1957) Effects of a podophyllotoxin derivative on tissue culture systems in which human cancer invades normal tissue. Cancer Res 17:336–344PubMedGoogle Scholar
  10. 10.
    Leighton J (1957) Contributions of tissue culture studies to an understanding of the biology of cancer: a review. Cancer Res 17:929–941PubMedGoogle Scholar
  11. 11.
    Slifkin M, Merkow LP, Pardo M, Epstein SM, Leighton J, Farber E (1970) Growth in vitro of cells from hyperplastic nodules of liver induced by 2-fluorenylacetamide or aflatoxin B1. Science 167:285–287CrossRefPubMedGoogle Scholar
  12. 12.
    Leighton J (1969) Propagation of cancer: targets for future chemotherapy. Cancer Res 29:2457–2465PubMedGoogle Scholar
  13. 13.
    Leighton J, Mark R, Justh G (1968) Patterns of three-dimensional growth in vitro in collagen-coated cellulose sponge: carcinomas and embryonic tissues. Cancer Res 28:286–296PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.AntiCancer, Inc.San DiegoUSA
  2. 2.Department of SurgeryUCSDSan DiegoUSA

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