Electrospinning Functionalized Polymers for Use as Tissue Engineering Scaffolds
Electrospinning polymers is a versatile technique to generate fibrous, three-dimensional scaffolds for tissue engineering applications. Modifying polymers with functional groups prior to electrospinning offers the opportunity to control the spatial presentation of functional groups within the scaffold as well as incorporate multiple bioactive cues. This chapter describes methods to modify poly(ε-caprolactone) (PCL) with peptides and electrospin these peptide-PCL conjugates to functionalize a scaffold surface in a single step. Methods to adapt standard electrospinning setups to create single- or dual-peptide gradients within a single construct are also described.
Key wordsTissue engineering Regenerative medicine Electrospinning Functionalized polymers Biodegradable scaffold Gradient biomaterials
This work was supported by start-up funds provided by Lehigh University. The author gratefully acknowledges helpful discussions about electrospinning with Dr. Geraldine Guex.