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Detection and Quantification of MicroRNAs in Esophageal Adenocarcinoma

  • Moein Amin
  • Farhadul Islam
  • Vinod Gopalan
  • Alfred K. Lam
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1756)

Abstract

MicroRNAs (miRNAs) are a class of small, noncoding RNAs that have been emerging as novel regulators in esophageal adenocarcinoma. Their role has been established in various aspects of esophageal adenocarcinoma including carcinogenesis, progression, treatment, and prognosis. Therefore, miRNA detection, profiling, and quantification have become extremely important for scientists and clinicians. As miRNAs are small, their detection can be challenging. There have been various methods developed to detect and/or quantify miRNAs. This chapter aims to introduce the fundamentals and methods of the most commonly used approaches including miRNA microarrays and quantitative real-time polymerase chain reaction (RT-qPCR) to detect and quantify miRNAs in esophageal adenocarcinoma.

Key words

MiRNA Esophageal adenocarcinoma Microarray Northern blotting RT-qPCR 

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Copyright information

© Springer Science+Business Media, LLC 2018

Authors and Affiliations

  • Moein Amin
    • 1
  • Farhadul Islam
    • 1
    • 2
  • Vinod Gopalan
    • 1
  • Alfred K. Lam
    • 1
  1. 1.Cancer Molecular Pathology of School of MedicineGriffith UniversityGold CoastAustralia
  2. 2.Department of Biochemistry and Molecular BiologyUniversity of RajshahiRajshahiBangladesh

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